To disable negative time off balances, you will want to visit Settings followed by Time Off:

On the Time Off page, you will need to select Edit next to the time off code you'd like to disable negative balances for: 

Uncheck the box next to Negative Balances Allowed and or disable the option on a per employee basis. Save once done:

If you'd like to disable negative balance for future employees, make sure to uncheck the box next to Allow negative balances for new employees:

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